Generator

Part:BBa_K1075051:Design

Designed by: Max Schelski   Group: iGEM13_Bonn   (2013-10-12)


pLac-RBS34-mazF-TT - inducible Killswitch


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We first constructed the part with two RBS of different strengths (B0034 and B0032). While the part with the weaker RBS (B0032) did not show the ability do inducible cell death this part (B0034) was cappable of IPTG inducible kill bacterial cells.


Source

mazF originally occurs in the genome of bacteria

References